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Mucin Gene Expression in Reflux Laryngeal Mucosa:Histological and In Situ Hybridization Observations

Lookup NU author(s): Dr David Bulmer, Professor Jeffrey Pearson

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

To determine if laryngopharyngeal reflux alters mucin gene expression in laryngeal mucosa. Methods. Insitu hybridization was employed to study the expression of the 8 well-characterised mucin genes MUC1-4, 5AC, 5B, 6, and 7 inreflux laryngeal mucosa from laryngeal ventricles, posterior commissures, and vocal folds compared to control/normal laryngealmucosa. Results. MUC1-5 genes are expressed in normal and reflux laryngeal mucosa. MUC1, 3 and 4 are expressed in respiratoryand squamous mucosa whereas MUC2 and 5AC are expressed in respiratory mucosa only. MUC3, 4 and 5AC are downregulatedin reflux mucosa. MUC5AC expression is significantly reduced in the 3 mucosal sites and when mucosal type was taken intoaccount, this remains significant in combined laryngeal and ventricular mucosa only. Conclusions. MUC3, 4 and 5AC expressionis downregulated in laryngopharyngeal reflux. This may be due to laryngeal mucosal metaplasia and/or alteration of mucin geneexpression in the preexisting mucosa. Altered mucin gene expression might predispose laryngeal mucosa to the damaging effect ofreflux

Publication metadata

Author(s): El-Sayed Ali M, Bulmer DM, Dettmar PW, Pearson JP

Publication type: Article

Publication status: Published

Journal: International Journal of Otolaryngology

Year: 2014

Volume: 2014

Print publication date: 24/03/2014

Online publication date: 24/03/2014

Acceptance date: 25/09/2013

Date deposited: 26/10/2022

ISSN (print): 1687-9201

ISSN (electronic): 1687-921X

Publisher: Hindawi Publishing Corporation

URL: https://doi.org/10.1155/2014/264075

DOI: 10.1155/2014/264075

PubMed id: 24790604

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