Toggle Main Menu Toggle Search

Open Access padlockePrints

A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates

Lookup NU author(s): Issac Goh



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2022 The Author(s)We present a multiomic cell atlas of human lung development that combines single-cell RNA and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging. Coupling single-cell methods with spatial analysis has allowed a comprehensive cellular survey of the epithelial, mesenchymal, endothelial, and erythrocyte/leukocyte compartments from 5–22 post-conception weeks. We identify previously uncharacterized cell states in all compartments. These include developmental-specific secretory progenitors and a subtype of neuroendocrine cell related to human small cell lung cancer. Our datasets are available through our web interface ( To illustrate its general utility, we use our cell atlas to generate predictions about cell-cell signaling and transcription factor hierarchies which we rigorously test using organoid models.

Publication metadata

Author(s): He P, Lim K, Sun D, Pett JP, Jeng Q, Polanski K, Dong Z, Bolt L, Richardson L, Mamanova L, Dabrowska M, Wilbrey-Clark A, Madissoon E, Tuong ZK, Dann E, Suo C, Goh I, Yoshida M, Nikolic MZ, Janes SM, He X, Barker RA, Teichmann SA, Marioni JC, Meyer KB, Rawlins EL

Publication type: Article

Publication status: Published

Journal: Cell

Year: 2022

Volume: 185

Issue: 25

Pages: 4841-4860.e25

Print publication date: 08/12/2022

Online publication date: 08/12/2022

Acceptance date: 03/11/2022

Date deposited: 05/01/2023

ISSN (print): 0092-8674

ISSN (electronic): 1097-4172

Publisher: Elsevier B.V.


DOI: 10.1016/j.cell.2022.11.005

PubMed id: 36493756


Altmetrics provided by Altmetric


Funder referenceFunder name
NIHR Biomedical Research Centre