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Analysis of glycans in a Burnt-on/Baked-on (BoBo) model food soil using Microarray Polymer Profiling (MAPP) and immunofluorescence microscopy

Lookup NU author(s): Cassie Bakshani, Dr Fiona Cuskin, Dr Hamish Yau, Professor William WillatsORCiD, Professor Grant Burgess



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2023 The AuthorsBurning of food materials during cooking can increase the difficulty in removal from solid surfaces, forming residual food soils. Using molecular probe-based technologies, the aim of this work was to elucidate the composition and relative abundance of glycans within a Burnt-On/Baked-On (BoBo) model food soil and investigate enzyme systems that may facilitate soil breakdown. Microarray Polymer Profiling identified xylan, arabinoxylan, mixed-linkage glucan and mannan as target substrates for the enzymatic cleaning of BoBo residues from surfaces. Indirect immunofluorescence microscopy revealed that burning resulted in extensive structural modifications and degradation of the three-dimensional architecture of constituent polysaccharide matrices. Results from high-throughput enzyme screening indicate that inclusion of xylan depolymerising enzymes in automatic dishwashing detergents may improve cleaning of recalcitrant, plant glycan-rich BoBo soils. Collectively, this study provides new insight into the composition and removal chemistry of complex, multi-component food soils.

Publication metadata

Author(s): Bakshani CR, Cuskin F, Lant NJ, Yau HCL, Willats WGT, Burgess J

Publication type: Article

Publication status: Published

Journal: Food Chemistry

Year: 2023

Volume: 410

Print publication date: 01/06/2023

Online publication date: 02/01/2023

Acceptance date: 31/12/2022

Date deposited: 24/01/2023

ISSN (print): 0308-8146

ISSN (electronic): 1873-7072

Publisher: Elsevier Ltd


DOI: 10.1016/j.foodchem.2022.135379

PubMed id: 36621331


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Funder referenceFunder name
Biotechnology and Biological Sciences Research Council BBSRC
BH14, 8792