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Methodological challenges in neonatal microbiome research

Lookup NU author(s): Dr Jon Chapman, Dr Christopher StewartORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Following microbial colonization at birth, the gut microbiome plays a vital role in the healthy development of human neonates and impacts both health and disease in later life. Understanding the development of the neonatal gut microbiome and how it interacts with the neonatal host are therefore important areas of study. However, research within this field must address a range of specific challenges that impact the design and implementation of research methods. If not considered ahead of time, these challenges have the potential to introduce biases into studies, negatively affecting the relevance, reproducibility, and impact of any findings. This review outlines the nature of these challenges and points to current and future solutions, as outlined in the literature, to assist researchers in the early stages of study design.

Publication metadata

Author(s): Chapman JA, Stewart CJ

Publication type: Review

Publication status: Published

Journal: Gut Microbes

Year: 2023

Volume: 15

Issue: 1

Online publication date: 26/02/2023

Acceptance date: 16/02/2023

ISSN (print): 1949-0976

ISSN (electronic): 1949-0984

Publisher: Taylor & Francis


DOI: 10.1080/19490976.2023.2183687

PubMed id: 36843005