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Lookup NU author(s): Cameron Robertson, Yuan Xue, Shobir Chowdhury, Dr Laura Maringele
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.Rif1 mediates telomere length, DNA replication, and DNA damage responses in budding yeast. Previous work identified several posttranslational modifications of Rif1, however none of these was shown to mediate the molecular or cellular responses to DNA damage, including telomere damage. We searched for such modifications using immunoblotting methods and the cdc13-1 and tlc1Δ models of telomere damage. We found that Rif1 is phosphorylated during telomere damage, and that serines 57 and 110 within a novel phospho-gate domain (PGD) of Rif1 are important for this modification, in cdc13-1 cells. The phosphorylation of Rif1 appeared to inhibit its accumulation on damaged chromosomes and the proliferation of cells with telomere damage. Moreover, we found that checkpoint kinases were upstream of this Rif1 phosphorylation and that the Cdk1 activity was essential for maintaining it. Apart from telomere damage, S57 and S110 were essential for Rif1 phosphorylation during the treatment of cells with genotoxic agents or during mitotic stress. We propose a speculative “Pliers” model to explain the role of the PGD phosphorylation during telomere and other types of damage.
Author(s): Robertson CM, Xue Y, Chowdhury S, Maringele L
Publication type: Article
Publication status: Published
Journal: Molecular and Cellular Biology
Year: 2023
Volume: 43
Issue: 5
Pages: 185-199
Online publication date: 04/05/2023
Acceptance date: 08/02/2023
Date deposited: 17/05/2023
ISSN (print): 0270-7306
ISSN (electronic): 1098-5549
Publisher: Taylor and Francis Ltd.
URL: https://doi.org/10.1080/10985549.2023.2193768
DOI: 10.1080/10985549.2023.2193768
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