Browse by author
Lookup NU author(s): Dr Rixia ZanORCiD, Adrian Blackburn, Dr Aom Plaimart, Dr Kishor AcharyaORCiD, Professor Claire Walsh, Dr Ross StirlingORCiD, Professor Chris Kilsby, Professor David WernerORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
There is no reference of microbiological water quality in the European Union's Water Framework Directive, adapted into English law, and consequently microbial water quality is not routinely monitored in English rivers, except for two recently designated bathing water sites. To address this knowledge gap, we developed an innovative monitoring approach for quantitative assessment of combined sewer overflow (CSO) impacts on the bacteriology of receiving rivers. Our approach combines conventional and environmental DNA (eDNA) based methods to generate multiple lines of evidence for assessing risks to public health. We demonstrated this approach by investigating spatiotemporal variation in the bacteriology of the Ouseburn in northeast England for different weather conditions in the summer and early autumn of the year 2021 across eight sampling locations that comprised rural, urban, and recreational land use settings. We characterized pollution source attributes by collecting sewage from treatment works and CSO discharge at the peak of a storm event. CSO discharge was characterized by log10 values per 100 mL (average ± stdev) of 5.12 ± 0.03 and 4.90 ± 0.03 for faecal coliforms and faecal streptococci, and 6.00 ± 0.11 and 7.78 ± 0.04 for rodA and HF183 genetic markers, for E. coli and human host associated Bacteroides, respectively, indicating about 5 % sewage content. SourceTracker analysis of sequencing data attributed 72–77 % of bacteria in the downstream section of the river during a storm event to CSO discharge sources, versus only 4–6 % to rural upstream sources. Data from sixteen summer sampling events in a public park exceeded various guideline values for recreational water quality. Quantitative microbial risk assessment (QMRA) predicted a median and 95th percentile risk of 0.03 and 0.39, respectively, of contracting a bacterial gastrointestinal disease when wading and splashing around in the Ouseburn. We show clearly why microbial water quality should be monitored where rivers flow through public parks, irrespective of their bathing water designation.
Author(s): Zan R, Blackburn A, Plaimart J, Acharya K, Walsh C, Stirling R, Kilsby C, Werner D
Publication type: Article
Publication status: Published
Journal: Science of The Total Environment
Year: 2023
Volume: 889
Print publication date: 01/09/2023
Online publication date: 18/05/2023
Acceptance date: 15/05/2023
Date deposited: 05/06/2023
ISSN (electronic): 0048-9697
Publisher: Elsevier
URL: https://doi.org/10.1016/j.scitotenv.2023.164282
DOI: 10.1016/j.scitotenv.2023.164282
Data Access Statement: Fastq files generated from 16S rRNA gene sequencing have been submitted to the NCBI Sequence Read Archive (SRA) with BioProject accession numbers PRJNA814853 (Ouseburn data) and PRJNA837409 (Birtley STP data). Additional data created during this research are openly available (https://doi.org/10.25405/data.ncl.22774688). Please contact Newcastle Research Data Service at rdm@ncl.ac.uk for access instructions.
Altmetrics provided by Altmetric
