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Lookup NU author(s): Dr Halah Ahmed, Sadat Aziz, Professor Marco Carrozzo, Professor Ruth Valentine
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2023 The Authors. Journal of Oral Pathology & Medicine published by John Wiley & Sons Ltd.Background: Most oral squamous cell carcinoma patients present with late-stage disease. Early detection of the disease is considered to be the most effective way of improving patient outcomes. Several biomarkers have been identified as indicators of oral cancer development and progression; however, none have been translated into clinical practice. In this study, we have investigated the role of Epsin3, an endocytic adaptor protein, and Notch1, a transmembrane signalling protein, in oral carcinogenesis with a view to explore their potential as biomarkers. Methods: Oral cancer cell lines and a normal oral keratinocyte cell line were used together with tissue samples of normal oral mucosa (n = 21), oral epithelial dysplasia (n = 74) and early stage (Stages I and II) oral squamous cell carcinoma (n = 31). Immunocytochemical staining, immunoblotting and real-time quantitative polymerase chain reaction (PCR) were performed to assess protein as well as gene expression levels. Results: The expression levels of Epsin3 and Notch1 mRNA and protein are variable across different oral squamous cell carcinoma derived cell lines. Epsin3 was upregulated in oral epithelial dysplasia and oral squamous cell carcinoma tissues compared with normal epithelium. Overexpression of Epsin3 resulted in a significant reduction of Notch1 expression in oral squamous cell carcinoma. Notch1 was generally downregulated in the dysplasia and oral squamous cell carcinoma samples. Conclusion: Epsin3 is upregulated in oral epithelial dysplasia and oral squamous cell carcinoma and has the potential to be used as a biomarker for oral epithelial dysplasia. Notch signalling is downregulated in oral squamous cell carcinoma, possibly through an Epsin3-induced de-activation pathway.
Author(s): Ahmed H, Paterson I, Aziz SA, Cremona O, Robinson M, Carrozzo M, Valentine RA
Publication type: Article
Publication status: Published
Journal: Journal of Oral Pathology and Medicine
Year: 2023
Volume: 52
Issue: 8
Pages: 710-717
Print publication date: 01/09/2023
Online publication date: 20/06/2023
Acceptance date: 26/05/2023
Date deposited: 12/07/2023
ISSN (print): 0904-2512
ISSN (electronic): 1600-0714
Publisher: John Wiley and Sons Inc
URL: https://doi.org/10.1111/jop.13460
DOI: 10.1111/jop.13460
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