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Biofilm Inhibitor Taurolithocholic Acid Alters Colony Morphology, Specialized Metabolism, and Virulence of Pseudomonas aeruginosa

Lookup NU author(s): Manuel Banzhaf

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Abstract

Copyright © 2019 American Chemical Society. Biofilm inhibition by exogenous molecules has been an attractive strategy for the development of novel therapeutics. We investigated the biofilm inhibitor taurolithocholic acid (TLCA) and its effects on the specialized metabolism, virulence, and biofilm formation of the clinically relevant bacterium Pseudomonas aeruginosa strain PA14. Our study shows that TLCA alters the specialized metabolism, thereby affecting P. aeruginosa colony biofilm physiology. We observed an upregulation of metabolites correlated to virulence such as the siderophore pyochelin. A wax moth virulence assay confirmed that treatment with TLCA increases the virulence of P. aeruginosa. On the basis of our results, we believe that future endeavors to identify biofilm inhibitors must consider how a putative lead alters the specialized metabolism of a bacterial community to prevent pathogens from entering a highly virulent state.

Publication metadata

Author(s): Condren AR, Kahl LJ, Boelter G, Kritikos G, Banzhaf M, Dietrich LEP, Sanchez LM

Publication type: Article

Publication status: Published

Journal: ACS Infectious Diseases

Year: 2020

Volume: 6

Issue: 4

Pages: 603-612

Print publication date: 10/04/2020

Online publication date: 18/12/2019

Acceptance date: 09/11/2019

ISSN (electronic): 2373-8227

Publisher: American Chemical Society

URL: https://doi.org/10.1021/acsinfecdis.9b00424

DOI: 10.1021/acsinfecdis.9b00424

PubMed id: 31851822

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