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Lookup NU author(s): Dr Kirsty McInnes
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2023, The Author(s).UV-B radiation regulates numerous morphogenic, biochemical and physiological responses in plants, and can stimulate some responses typically associated with other abiotic and biotic stimuli, including invertebrate herbivory. Removal of UV-B from the growing environment of various plant species has been found to increase their susceptibility to consumption by invertebrate pests, however, to date, little research has been conducted to investigate the effects of UV-B on crop susceptibility to field pests. Here, we report findings from a multi-omic and genetic-based study investigating the mechanisms of UV-B-stimulated resistance of the crop, Brassica napus (oilseed rape), to herbivory from an economically important lepidopteran specialist of the Brassicaceae, Plutella xylostella (diamondback moth). The UV-B photoreceptor, UV RESISTANCE LOCUS 8 (UVR8), was not found to mediate resistance to this pest. RNA-Seq and untargeted metabolomics identified components of the sinapate/lignin biosynthetic pathway that were similarly regulated by UV-B and herbivory. Arabidopsis mutants in genes encoding two enzymes in the sinapate/lignin biosynthetic pathway, CAFFEATE O-METHYLTRANSFERASE 1 (COMT1) and ELICITOR-ACTIVATED GENE 3–2 (ELI3-2), retained UV-B-mediated resistance to P. xylostella herbivory. However, the overexpression of B. napus COMT1 in Arabidopsis further reduced plant susceptibility to P. xylostella herbivory in a UV-B-dependent manner. These findings demonstrate that overexpression of a component of the sinapate/lignin biosynthetic pathway in a member of the Brassicaceae can enhance UV-B-stimulated resistance to herbivory from P. xylostella. Graphical abstract: [Figure not available: see fulltext.].
Author(s): McInnes KJ, van der Hooft JJJ, Sharma A, Herzyk P, Hundleby PAC, Schoonbeek H-J, Amtmann A, Ridout C, Jenkins GI
Publication type: Article
Publication status: Published
Journal: Photochemical and Photobiological Sciences
Year: 2023
Volume: 22
Pages: 2341-2356
Online publication date: 28/07/2023
Acceptance date: 04/07/2023
Date deposited: 08/09/2023
ISSN (print): 1474-905X
ISSN (electronic): 1474-9092
Publisher: Springer Nature
URL: https://doi.org/10.1007/s43630-023-00455-9
DOI: 10.1007/s43630-023-00455-9
Data Access Statement: The RNA-Seq dataset generated and analysed in this study has been deposited in the National Center for Biotechnology Information’s Gene Expression Omnibus (GEO) database (GEO Series accession number GSE239314, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE239314). The raw metabolomic data have been deposited in MetaboLights Data Repository under the study identifier code MTBLS8236. Other data generated in this study are included in this article and its supplementary information files.
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