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Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution

Lookup NU author(s): Dr Ryley Parrish, Professor Andrew Trevelyan



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2023 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society. We recently reported that strong activation of the optogenetic chloride pump, halorhodopsin leads to a secondary redistribution of K+ ions into the cell, through tonically open, “leak” K+ channels. Here we show that this effect is not unique to halorhodopsin but is also seen with activation of another electrogenic ion pump, archaerhodopsin. The two opsins differ however in the size of the rebound rise in extracellular potassium, [K+]o, after the end of activation, which is far larger with halorhodopsin than for archaerhodopsin activation. Multiple linear regression modeling indicates that the variance in the postillumination surge in [K+]o was explained both by the size of the preceding, illumination-induced drop in [K+]o and also by the type of opsin. These data provide additional support for the hypothesis that intense chloride-loading of cells, as occurs naturally following intense bursts of GABAergic synaptic bombardment, or artificially following halorhodopsin activation, is followed by extrusion of both Cl− and K+ coupled together. We discuss this with respect to the pattern of [K+]o rise that occurs at the onset of seizure-like events.

Publication metadata

Author(s): Parrish RR, Jackson-Taylor T, Voipio J, Trevelyan AJ

Publication type: Article

Publication status: Published

Journal: Physiological Reports

Year: 2023

Volume: 11

Issue: 15

Online publication date: 03/08/2023

Acceptance date: 11/07/2023

Date deposited: 13/09/2023

ISSN (print): 2051-817X

Publisher: John Wiley & Sons Ltd


DOI: 10.14814/phy2.15778

PubMed id: 37537145


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Funder referenceFunder name
BB/P019854/1Biotechnology and Biological Sciences Research Council (BBSRC)
MR/R005427/1Medical Research Council (MRC)