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Lookup NU author(s): Cassie Bakshani, Sarana Sommano, Professor William WillatsORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2023 JoVE. Microarray polymer profiling (MAPP) is a robust and reproducible approach to systematically determine the composition and relative abundance of glycans and glycoconjugates within a variety of biological samples, including plant and algal tissues, food materials, and human, animal, and microbial samples. Microarray technology underpins the efficacy of this method by providing a miniaturized, high-throughput screening platform, allowing thousands of interactions between glycans and highly specific glycan-directed molecular probes to be characterized concomitantly, using only small amounts of analytes. Constituent glycans are chemically and enzymatically fractionated, before being sequentially extracted from the sample and directly immobilized onto nitrocellulose membranes. The glycan composition is determined by the attachment of specific glycan-recognizing molecular probes to the extorted and printed molecules. MAPP is complementary to conventional glycan analysis techniques, such as monosaccharide and linkage analysis and mass spectrometry. However, glycan-recognizing molecular probes provide insight into the structural configurations of glycans, which can aid in elucidating biological interactions and functional roles.
Author(s): Bakshani CR, Sangta J, Sommano S, Willats WGT
Publication type: Article
Publication status: Published
Journal: Journal of Visualized Experiments
Year: 2023
Volume: 2023
Issue: 199
Online publication date: 29/09/2023
Acceptance date: 02/04/2018
Date deposited: 09/11/2023
ISSN (electronic): 1940-087X
Publisher: Journal of Visualized Experiments
URL: https://doi.org/10.3791/65443
DOI: 10.3791/65443
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