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2-DE-based and shotgun proteomics approach in the analysis of the seed proteome of a low phytic acid rice (Oryza sativa, ssp. japonica) mutant

Lookup NU author(s): Dr Kaveh Emami, Dr Ahmad Al-Mrabeh, Professor Angharad MR GatehouseORCiD

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Abstract

© 2023 Elsevier LtdA low phytic acid (lpa) rice mutant line, denoted as Os-lpa-XS110-2, was developed through gamma irradiation (Liu et al., 2007). Subsequently, this mutant line was cultivated alongside its parental wild-type counterpart, Xiushui 110, in four distinct field trials conducted in China. The primary objective of this research was to conduct a comparative proteomic analysis between the parental and mutant lines, aiming to detect and compare protein species with varying levels of relative abundance and their potential involvement in phytic acid biosynthesis. To elucidate the molecular underpinnings of this alteration, proteome analyses were conducted on both the parental and mutant lines, employing LC-MS/MS in conjunction with 2-dimensional gel electrophoresis. Only, 7 proteins consistently exhibited significant differential abundance across all trial locations, suggesting that the regulation of these proteins is less susceptible to environmental influences. In a broader context, among the 700 detected proteins, 56 displayed noteworthy up- or down-regulation across diverse geographical regions. Notably, storage and housekeeping proteins emerged as the most frequently identified protein categories, with globulin and glutelin demonstrating prominent differences in abundance. However, based on our current understanding, none of the identified proteins appear to have direct roles in phytic acid biosynthesis.

Publication metadata

Author(s): Emami K, Mesbahi E, Al-Mrabeh A, Gatehouse AMR, Shu Q-Y, Golebiowska-Paluch G

Publication type: Article

Publication status: Published

Journal: Journal of Cereal Science

Year: 2023

Volume: 114

Print publication date: 01/11/2023

Online publication date: 17/10/2023

Acceptance date: 17/10/2023

ISSN (print): 0733-5210

ISSN (electronic): 1095-9963

Publisher: Academic Press

URL: https://doi.org/10.1016/j.jcs.2023.103801

DOI: 10.1016/j.jcs.2023.103801

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