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A type VII-secreted lipase toxin with reverse domain arrangement

Lookup NU author(s): Stephen Garrett, Dr Nicole Mietrach, Yaping Yang, Dr Fatima Ulhuq, Professor Tracy Palmer FRS FRSE FMedSciORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2023, The Author(s). The type VII protein secretion system (T7SS) is found in many Gram-positive bacteria and in pathogenic mycobacteria. All T7SS substrate proteins described to date share a common helical domain architecture at the N-terminus that typically interacts with other helical partner proteins, forming a composite signal sequence for targeting to the T7SS. The C-terminal domains are functionally diverse and in Gram-positive bacteria such as Staphylococcus aureus often specify toxic anti-bacterial activity. Here we describe the first example of a class of T7 substrate, TslA, that has a reverse domain organisation. TslA is widely found across Bacillota including Staphylococcus, Enterococcus and Listeria. We show that the S. aureus TslA N-terminal domain is a phospholipase A with anti-staphylococcal activity that is neutralised by the immunity lipoprotein TilA. Two small helical partner proteins, TlaA1 and TlaA2 are essential for T7-dependent secretion of TslA and at least one of these interacts with the TslA C-terminal domain to form a helical stack. Cryo-EM analysis of purified TslA complexes indicate that they share structural similarity with canonical T7 substrates. Our findings suggest that the T7SS has the capacity to recognise a secretion signal present at either end of a substrate.


Publication metadata

Author(s): Garrett SR, Mietrach N, Deme J, Bitzer A, Yang Y, Ulhuq FR, Kretschmer D, Heilbronner S, Smith TK, Lea SM, Palmer T

Publication type: Article

Publication status: Published

Journal: Nature Communications

Year: 2023

Volume: 14

Issue: 1

Online publication date: 19/12/2023

Acceptance date: 05/12/2023

Date deposited: 26/01/2024

ISSN (electronic): 2041-1723

Publisher: Springer Nature

URL: https://doi.org/10.1038/s41467-023-44221-y

DOI: 10.1038/s41467-023-44221-y

Data Access Statement: Other data generated or analysed in this study are available within the article and its supplementary materials. Source data are provided with this paper with the exception of the microscopy images which are available at FigShare (https://doi.org/10.6084/m9.figshare.24717864.v1). Databases used are Alphafold (https://alphafold.ebi.ac.uk/), Refseq (https://www.ncbi.nlm.nih.gov/refseq/) and Lipid maps (https://www.lipidmaps.org). Source data are provided with this paper.

PubMed id: 38114483


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Funding

Funder referenceFunder name
10183/Z/15/Z
224151/Z/21/Z
China Scholarship Council
BB/M011186/1
BBSRC
DFG (German Research Foundation)
German Centre of Infection Research (DZIF)
Intramural Research Program of the NIH, NCI, Center for Cancer Research
M2871/1-1
Wellcome Trust
TTU 08.708

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