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Lookup NU author(s): Dr Chris DerrickORCiD, Dr Ahlam Alqahtani, Dr Lorraine Eley, Professor Deborah HendersonORCiD, Dr Bill Chaudhry
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© The Author(s) 2023. Published by Oxford University Press. Developmental studies have shown that the evolutionarily conserved Wnt Planar Cell Polarity (PCP) pathway is essential for the development of a diverse range of tissues and organs including the brain, spinal cord, heart and sensory organs, as well as establishment of the left-right body axis. Germline mutations in the highly conserved PCP gene VANGL2 in humans have only been associated with central nervous system malformations, and functional testing to understand variant impact has not been performed. Here we report three new families with missense variants in VANGL2 associated with heterotaxy and congenital heart disease p.(Arg169His), non-syndromic hearing loss p.(Glu465Ala) and congenital heart disease with brain defects p.(Arg135Trp). To test the in vivo impact of these and previously described variants, we have established clinically-relevant assays using mRNA rescue of the vangl2 mutant zebrafish. We show that all variants disrupt Vangl2 function, although to different extents and depending on the developmental process. We also begin to identify that different VANGL2 missense variants may be haploinsufficient and discuss evidence in support of pathogenicity. Together, this study demonstrates that zebrafish present a suitable pipeline to investigate variants of unknown significance and suggests new avenues for investigation of the different developmental contexts of VANGL2 function that are clinically meaningful.
Author(s): Derrick CJ, Szenker-Ravi E, Santos-Ledo A, Alqahtani A, Yusof A, Eley L, Coleman AHL, Tohari S, Ng AY-J, Venkatesh B, Alharby E, Mansard L, Bonnet-Dupeyron M-N, Roux A-F, Vache C, Roume J, Bouvagnet P, Almontashiri NAM, Henderson DJ, Reversade B, Chaudhry B
Publication type: Article
Publication status: Published
Journal: Human Molecular Genetics
Year: 2024
Volume: 33
Issue: 2
Pages: 150-169
Print publication date: 15/01/2024
Online publication date: 10/10/2023
Acceptance date: 28/09/2023
Date deposited: 23/01/2024
ISSN (print): 0964-6906
ISSN (electronic): 1460-2083
Publisher: Oxford University Press
URL: https://doi.org/10.1093/hmg/ddad171
DOI: 10.1093/hmg/ddad171
Data Access Statement: All data that supports the findings not presented in main figures and supplementary files are available from the relevant corresponding author upon request.
PubMed id: 37815931
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