Toggle Main Menu Toggle Search

Open Access padlockePrints

Microbacterium gubbeenense sp nov., from the surface of a smear-ripened cheese

Lookup NU author(s): Professor Michael Goodfellow, Emeritus Professor Alan Ward


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Phenotypic and phylogenetic studies were performed on 11 strains of a Microbacterium-like organism isolated from the surface of a smear-ripened cheese. The isolates were Gram-positive, catalase-positive, facultatively anaerobic, oxidase-negative, non-spore-forming, non-motile, small, slender rods and grew in 12% (w/v) NaCl. Chemotaxonomic investigation revealed that all the isolates belonged unambiguously to the genus Microbacterium. They contained type B1 peptidoglycans with L-lysine as the diamino acid and glycolyl acyl types; rhamnose and galactose were the cell wall sugars. The G+C content ranged from 69 to 72 mol%. The major menaquinones were MK-11 and MK-12 and the major fatty acids were anteiso C15:0 and C17:0 and iso C16:0. Phylogenetic analysis of the 16S rRNA sequences of four isolates showed that they represented a new subline in the genus Microbacterium, with Microbacterium barkeri as their nearest phylogenetic neighbour. M. barkeri showed the highest sequence similarity to the isolates; however, DNA-DNA hybridization showed that the isolates had only 38% chromosomal similarity to M. barkeri. Based on the phylogenetic and phenotypic distinctiveness of the isolates, it is proposed that they be classified as a new Microbacterium species, for which the name Microbacterium gubbeenense sp. nov. is suggested. The type strain has been deposited as LMG S-19263(T) (= NCIMB 30129(T)). The GenBank accession number for the 16S rDNA sequence of the type strain is AF263563.

Publication metadata

Author(s): Ward AC; Goodfellow M; Brennan NM; Brown R; Beresford TP; Vancanneyt M; Cogan TM; Fox PF

Publication type: Article

Publication status: Published

Journal: International Journal of Systematic and Evolutionary Microbiology

Year: 2001

Volume: 51

Issue: 6

Pages: 1969-1976

ISSN (print): 1466-5026

ISSN (electronic): 1466-5034

Publisher: Society for General Microbiology


DOI: 10.1099/00207713-51-6-1969

Notes: Times Cited: 7 Part 6


Altmetrics provided by Altmetric