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Sequential action of JNK genes establishes the embryonic left-right axis

Lookup NU author(s): Dr Chris DerrickORCiD, Dr Adrian Santos, Dr Lorraine Eley, Bela Paramita, Professor Deborah HendersonORCiD, Dr Bill Chaudhry



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2022. Published by The Company of Biologists LtdThe establishment of the left-right axis is crucial for the placement, morphogenesis and function of internal organs. Left-right specification is proposed to be dependent on cilia-driven fluid flow in the embryonic node. Planar cell polarity (PCP) signalling is crucial for patterning of nodal cilia, yet downstream effectors driving this process remain elusive. We have examined the role of the JNK gene family, a proposed downstream component of PCP signalling, in the development and function of the zebrafish node. We show jnk1 and jnk2 specify length of nodal cilia, generate flow in the node and restrict southpaw to the left lateral plate mesoderm. Moreover, loss of asymmetric southpaw expression does not result in disturbances to asymmetric organ placement, supporting a model in which nodal flow may be dispensable for organ laterality. Later, jnk3 is required to restrict pitx2c expression to the left side and permit correct endodermal organ placement. This work uncovers multiple roles for the JNK gene family acting at different points during left-right axis establishment. It highlights extensive redundancy and indicates JNK activity is distinct from the PCP signalling pathway.

Publication metadata

Author(s): Derrick CJ, Santos-Ledo A, Eley L, Paramita IA, Henderson DJ, Chaudhry B

Publication type: Article

Publication status: Published

Journal: Development (Cambridge)

Year: 2022

Volume: 149

Issue: 9

Online publication date: 03/05/2022

Acceptance date: 09/03/2022

Date deposited: 13/02/2024

ISSN (print): 0950-1991

ISSN (electronic): 1477-9129

Publisher: Company of Biologists Ltd


DOI: 10.1242/dev.200136

PubMed id: 35352808


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Funder referenceFunder name
PG/15/58/31611British Heart Foundation