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Lookup NU author(s): Dr Chris DerrickORCiD, Dr Adrian Santos, Dr Lorraine Eley, Bela Paramita, Professor Deborah HendersonORCiD, Dr Bill Chaudhry
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2022. Published by The Company of Biologists LtdThe establishment of the left-right axis is crucial for the placement, morphogenesis and function of internal organs. Left-right specification is proposed to be dependent on cilia-driven fluid flow in the embryonic node. Planar cell polarity (PCP) signalling is crucial for patterning of nodal cilia, yet downstream effectors driving this process remain elusive. We have examined the role of the JNK gene family, a proposed downstream component of PCP signalling, in the development and function of the zebrafish node. We show jnk1 and jnk2 specify length of nodal cilia, generate flow in the node and restrict southpaw to the left lateral plate mesoderm. Moreover, loss of asymmetric southpaw expression does not result in disturbances to asymmetric organ placement, supporting a model in which nodal flow may be dispensable for organ laterality. Later, jnk3 is required to restrict pitx2c expression to the left side and permit correct endodermal organ placement. This work uncovers multiple roles for the JNK gene family acting at different points during left-right axis establishment. It highlights extensive redundancy and indicates JNK activity is distinct from the PCP signalling pathway.
Author(s): Derrick CJ, Santos-Ledo A, Eley L, Paramita IA, Henderson DJ, Chaudhry B
Publication type: Article
Publication status: Published
Journal: Development (Cambridge)
Year: 2022
Volume: 149
Issue: 9
Online publication date: 03/05/2022
Acceptance date: 09/03/2022
Date deposited: 13/02/2024
ISSN (print): 0950-1991
ISSN (electronic): 1477-9129
Publisher: Company of Biologists Ltd
URL: https://doi.org/10.1242/dev.200136
DOI: 10.1242/dev.200136
PubMed id: 35352808
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