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The zinc bound form of the actinomycete derived natural product compound JBIR-141 induces a mitotic phenotype in fission yeast

Lookup NU author(s): Dr Michael HallORCiD, Professor Jeff Errington

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Abstract

This study was designed to identify the natural product compound produced by the actinomycete strain “S149” which is capable of inducing a strong small cell phenotype, called wee, in the fission yeast Schizosaccharomyces pombe. We purified the bioactive molecule, which, on the basis of mass spectrometry data, was identified as a novel zinc-bound form of the previously published molecule JBIR-141. JBIR-141 was not previously known to be a zincophore and does not possess structural features common to other bacterial zinc-binding natural product compounds. Testing the effect of the Zn²⁺ bound form of JBIR-14 against a series of S. pombe deletion mutants which express a wee phenotype suggested its potential target in fission yeast is Pyp1, which is involved in regulation of the onset of mitosis, an important control in the eukaryotic cell cycle.

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Author(s): Lewis RA, Hayles J, Hall MJ, Gray J, Mebrate S, Li J, Allenby NEE, Errington J

Publication type: Article

Publication status: Published

Journal: Scientific Reports

Year: 2025

Volume: 15

Online publication date: 12/11/2025

Acceptance date: 06/10/2025

Date deposited: 20/11/2025

ISSN (electronic): 2045-2322

Publisher: Nature Publishing Group

URL: https://doi.org/10.1038/s41598-025-23271-w

DOI: 10.1038/s41598-025-23271-w

Data Access Statement: Data and materials relating to this study will be made available on request. Queries should be directed to Prof. J. Errington.

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