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Monitoring the Interaction of the Peptidoglycan with the Bacterial β-Barrel Assembly Machinery

Lookup NU author(s): Federico Corona, Professor Waldemar Vollmer


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© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. Gram-negative bacteria coordinate the biosynthesis of their different cell envelope components. Growth of the outer membrane (OM) requires the essential β-barrel assembly machine (BAM), which inserts OM proteins (OMPs) into the OM. The underlying peptidoglycan (PG) sacculus grows by the insertion of nascent glycan chains. We have previously identified interactions between BAM and PG in E. coli and showed that these interactions coordinate OM biogenesis with PG growth. BAM responds to the maturation state of the PG, and this mechanism activates preferentially BAM complexes at sites of active PG synthesis. Here we present protocols to purify soluble Bam proteins and full-length BamABCDE, isolate PG and soluble PG fragments, and study BAM-PG interactions with the isolated components. We also describe the protocol to detect interactions between Bam proteins and PG in cells.

Publication metadata

Author(s): Corona F, Vollmer W

Editor(s): Raffaele Ieva

Publication type: Book Chapter

Publication status: Published

Book Title: Transmembrane β-Barrel Proteins: Methods and Protocols

Year: 2024

Volume: 2778

Pages: 159-183

Print publication date: 14/03/2024

Online publication date: 14/03/2024

Acceptance date: 02/04/2018

Series Title: Methods in Molecular Biology

Publisher: Humana

Place Published: New York


DOI: 10.1007/978-1-0716-3734-0_11

PubMed id: 38478278

Library holdings: Search Newcastle University Library for this item

ISBN: 9781071637333