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Lookup NU author(s): Federico Corona, Professor Waldemar Vollmer
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© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. Gram-negative bacteria coordinate the biosynthesis of their different cell envelope components. Growth of the outer membrane (OM) requires the essential β-barrel assembly machine (BAM), which inserts OM proteins (OMPs) into the OM. The underlying peptidoglycan (PG) sacculus grows by the insertion of nascent glycan chains. We have previously identified interactions between BAM and PG in E. coli and showed that these interactions coordinate OM biogenesis with PG growth. BAM responds to the maturation state of the PG, and this mechanism activates preferentially BAM complexes at sites of active PG synthesis. Here we present protocols to purify soluble Bam proteins and full-length BamABCDE, isolate PG and soluble PG fragments, and study BAM-PG interactions with the isolated components. We also describe the protocol to detect interactions between Bam proteins and PG in cells.
Author(s): Corona F, Vollmer W
Editor(s): Raffaele Ieva
Publication type: Book Chapter
Publication status: Published
Book Title: Transmembrane β-Barrel Proteins: Methods and Protocols
Year: 2024
Volume: 2778
Pages: 159-183
Print publication date: 14/03/2024
Online publication date: 14/03/2024
Acceptance date: 02/04/2018
Series Title: Methods in Molecular Biology
Publisher: Humana
Place Published: New York
URL: https://doi.org/10.1007/978-1-0716-3734-0_11
DOI: 10.1007/978-1-0716-3734-0_11
PubMed id: 38478278
Library holdings: Search Newcastle University Library for this item
ISBN: 9781071637333