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Lookup NU author(s): Dr David Burns, Dr Rolando Berlinguer PalminiORCiD, Dr Andreas Werner
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© The Author(s) 2024. Phosphate (Pi) is an essential nutrient, and its plasma levels are under tight hormonal control. Uphill transport of Pi into cells is mediated by the two Na-dependent Pi transporter families SLC34 and SLC20. The molecular identity of a potential Pi export pathway is controversial, though XPR1 has recently been suggested by Giovannini and coworkers to mediate Pi export. We expressed XPR1 in Xenopus oocytes to determine its functional characteristics. Xenopus isoforms of proteins were used to avoid species incompatibility. Protein tagging confirmed the localization of XPR1 at the plasma membrane. Efflux experiments, however, failed to detect translocation of Pi attributable to XPR1. We tested various counter ions and export medium compositions (pH, plasma) as well as potential protein co-factors that could stimulate the activity of XPR1, though without success. Expression of truncated XPR1 constructs and individual domains of XPR1 (SPX, transmembrane core, C-terminus) demonstrated downregulation of the uptake of Pi mediated by the C-terminal domain of XPR1. Tethering the C-terminus to the transmembrane core changed the kinetics of the inhibition and the presence of the SPX domain blunted the inhibitory effect. Our observations suggest a regulatory role of XPR1 in cellular Pi handling rather than a function as Pi exporter. Accordingly, XPR1 senses intracellular Pi levels via its SPX domain and downregulates cellular Pi uptake via the C-terminal domain. The molecular identity of a potential Pi export protein remains therefore elusive.
Author(s): Burns D, Berlinguer-Palmini R, Werner A
Publication type: Article
Publication status: Published
Journal: Pflügers Archiv - European Journal of Physiology
Year: 2024
Volume: 476
Pages: 861-869
Online publication date: 20/03/2024
Acceptance date: 04/03/2024
Date deposited: 02/04/2024
ISSN (print): 0031-6768
ISSN (electronic): 1432-2013
Publisher: Springer Nature
URL: https://doi.org/10.1007/s00424-024-02941-0
DOI: 10.1007/s00424-024-02941-0
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