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Technical note: successful DNA amplification of DNA from non-destructive buccal swabbing in Vespertilionid and Rhinolophid bats

Lookup NU author(s): Dr Simon MaddockORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

© The Author(s) 2024.Acquiring DNA from wild bats (Mammalia: Chiroptera) is typically undertaken utilizing highly invasive (but non-lethal) sampling techniques comprising wing biopsies and occasional blood samples. While non-invasive sampling is possible through the extraction of DNA from faecal samples, it is not always possible to acquire samples from individual bats whilst conducting fieldwork, and as such, this method is primarily applicable to roost occupancy identification. Similarly, wing swabbing is liable to cross-contamination from roost mates. Here we present the first use of oral (buccal) swabbing for successful, species-resolution DNA sequencing of Vespertilionidae and Rhinolophidae in 10 bat species (nine Vespertilionidae and one Rhinolophidae) from the UK.

Publication metadata

Author(s): Hughes M, Brown SK, Martin R, Young CH, Maddock S

Publication type: Article

Publication status: Published

Journal: Conservation Genetics Resources

Year: 2024

Pages: epub ahead of print

Online publication date: 22/03/2024

Acceptance date: 19/02/2024

Date deposited: 04/04/2024

ISSN (print): 1877-7252

ISSN (electronic): 1877-7260

Publisher: Springer Science and Business Media B.V.

URL: https://doi.org/10.1007/s12686-024-01352-8

DOI: 10.1007/s12686-024-01352-8

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