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Lookup NU author(s): Professor Hans-Peter Klenk, Dr Maria Del Carmen Montero-CalasanzORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2024 The Authors.The taxonomic position of strain BMG 8361T, isolated from sandstone collected in the Sahara Desert of Southern Tunisia, was refined through a polyphasic taxonomic investigation. Colonies of BMG 8361T were pale-orange coloured, irregular with a dry surface and produced a diffusible pink or brown pigment depending on media. The Gram-positive cells were catalase-positive and oxidase-negative. The strain exhibited growth at 10–40°C and pH values ranging from 5.5 to 9.0, with optima at 28–35°C and pH 6.5–8.0. Additionally, BMG 8361T demonstrated the ability to grow in the presence of up to 1% NaCl (w/v) concentration. The peptidoglycan of the cell wall contained meso-diaminopimelic acid, glucose, galactose, xylose, ribose, and rhamnose. The predominant menaquinones consisted of MK-9(H4) and MK-9. The main polar lipids were phosphatidylcholine, phosphatidylinositol, glycophosphatidylinositol, diphosphatidylglycerol, phosphatidylethanolamine, and two unidentified lipids. Major cellular fatty acids were iso-C16:0, iso-C16:1 h, and C17:1 ω8c. Phylogenetic analyses based on both the 16S rRNA gene and whole-genome sequences assigned strain BMG 8361T within the genus Blastococcus. The highest pairwise sequence similarity observed in the 16S rRNA gene was 99.5% with Blastococcus haudaquaticus AT 7-14T. However, when considering digital DNA–DNA hybridization and average nucleotide identity, the highest values, 48.4 and 86.58%, respectively, were obtained with Blastococcus colisei BMG 822T. These values significantly undershoot the recommended thresholds for establishing new species, corroborating the robust support for the distinctive taxonomic status of strain BMG 8361T within the genus Blastococcus. In conjunction with the phenotyping results, this compelling evidence leads to the proposal of a novel species we named Blastococcus brunescens sp. nov. with BMG 8361T (=DSM 46845T=CECT 8880T) as the type strain.
Author(s): Hezbri K, Kammoun I, Sbissi I, Klenk H-P, Montero-Calasanz MC, Ghodhbane-Gtari F, Gtari M
Publication type: Article
Publication status: Published
Journal: International Journal of Systematic and Evolutionary Microbiology
Year: 2024
Volume: 74
Issue: 4
Online publication date: 03/04/2024
Acceptance date: 02/04/2024
Date deposited: 01/05/2024
ISSN (print): 1466-5026
ISSN (electronic): 1466-5034
Publisher: Microbiology Society
URL: https://doi.org/10.1099/ijsem.0.006317
DOI: 10.1099/ijsem.0.006317
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