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Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles

Lookup NU author(s): Professor Moein MoghimiORCiD

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Abstract

© 2024 Elsevier B.V.Complement plays a critical role in the immune response toward nanomaterials. The complement attack on a foreign surface results in the deposition of C3, assembly of C3 convertases, the release of anaphylatoxins C3a and C5a, and finally, the formation of membrane attack complex C5b-9. Various technologies can measure complement activation markers in the fluid phase, but measurements of surface C3 deposition are less common. Previously, we developed an ultracentrifugation-based dot blot immunoassay (DBI) to measure the deposition of C3 and other protein corona components on nanoparticles. Here, we validate the repeatability of the DBI and its correlation with pathway-specific and common fluid phase markers. Moreover, we discuss the advantages of DBI, such as cost-effectiveness and versatility, while addressing potential limitations. This study provides insights into complement activation at the nanosurface level, offering a valuable tool for nanomedicine researchers in the field.

Publication metadata

Author(s): Li Y, Monte A, Dylla L, Moghimi SM, Simberg D

Publication type: Article

Publication status: Published

Journal: Journal of Immunological Methods

Year: 2024

Volume: 528

Print publication date: 01/05/2024

Online publication date: 02/04/2024

Acceptance date: 01/04/2024

ISSN (print): 0022-1759

ISSN (electronic): 1872-7905

Publisher: Elsevier B.V.

URL: https://doi.org/10.1016/j.jim.2024.113668

DOI: 10.1016/j.jim.2024.113668

PubMed id: 38574804

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