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Lookup NU author(s): Dr Yi Zhang, Dr Kheng-Lim GohORCiD, Dr Vladimir Zivkovic, Dr Yuen Ling Ng
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2024 Elsevier LtdRaspberry ketone (RK) is a high-value bioactive component in the growing demand for natural compounds in food and flavour industries. However, limited production persists due to low-yield microorganisms and complex biosynthetic pathway. Here, an Nidula niveo-tomentosa fungi-based fermentation system was developed to investigate and optimize the cultivation parameters for natural RK production. While optimizing glucose and phenylalanine concentrations ensured sufficient substrate supply, it unexpectedly induced stress on the substrates. Despite having minimal impact on biomass growth, UV-A radiation effectively boosted RK biosynthesis, but also reaching a ceiling with extended exposure times. Younger precultures exhibited higher specific productivity, older cultures ultimately yielded more RK due to their higher inoculum concentration. Mycelial fungi, compared to fungal pellets, outperformed in RK production, likely facilitated by enhanced nutrient uptake and bioproduct transport. Ultimately, the combination of optimized parameters unlocked a peak RK yield of 154.4 mg/L, underscoring the remarkable potential for natural RK production.
Author(s): Zhang Y, Goh K-L, Zivkovic V, Ng YL, Chow Y
Publication type: Article
Publication status: Published
Journal: Process Biochemistry
Year: 2024
Volume: 144
Pages: 168-178
Print publication date: 01/09/2024
Online publication date: 31/05/2024
Acceptance date: 30/05/2024
Date deposited: 12/06/2024
ISSN (print): 1359-5113
ISSN (electronic): 1873-3298
Publisher: Elsevier Ltd
URL: https://doi.org/10.1016/j.procbio.2024.05.029
DOI: 10.1016/j.procbio.2024.05.029
ePrints DOI: 10.57711/ehqe-fs51
Data Access Statement: Data will be made available on request.
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