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Lookup NU author(s): Csenge Gal, Grace Cochrane, Professor Brian Morgan, Dr Simon Whitehall
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Quiescence (G0) is a reversible non-dividing state that facilitates cellular survival in adverse conditions. Here, we demonstrate that the HIRA histone chaperone complex is required for the reversibility and longevity of nitrogen starvation-induced quiescence in Schizosaccharomyces pombe. The HIRA protein, Hip1 is not required for entry into G0 or the induction of autophagy. Although hip1Δ cells retain metabolic activity in G0, they rapidly lose the ability to resume proliferation. After a short period in G0 (1 day), hip1Δ mutants can resume cell growth in response to the restoration of a nitrogen source but do not efficiently reenter the vegetative cell cycle. This correlates with a failure to induce the expression of MBF transcription factor-dependent genes that are critical for S phase. In addition, hip1Δ G0 cells rapidly progress to a senescent state in which they can no longer re-initiate growth following nitrogen source restoration. Analysis of a conditional hip1 allele is consistent with these findings and indicates that HIRA is required for efficient exit from quiescence and prevents an irreversible cell cycle arrest.
Author(s): Gal C, Cochrane GA, Morgan BA, Rallis C, Bähler J, Whitehall SK
Publication type: Article
Publication status: Published
Journal: Cell Cycle
Year: 2023
Volume: 22
Issue: 17
Pages: 1921-1936
Print publication date: 27/08/2023
Online publication date: 27/08/2023
Acceptance date: 31/08/2023
Date deposited: 28/06/2024
ISSN (print): 1538-4101
ISSN (electronic): 1551-4005
Publisher: Taylor & Francis
URL: https://doi.org/10.1080/15384101.2023.2249705
DOI: 10.1080/15384101.2023.2249705
Data Access Statement: Authors agree to make data and materials supporting the results or analyses presented in their paper available upon reasonable request. RNA-seq data have been submitted to GEO (accession number: GSE129599).
PubMed id: 37635373
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