Browse by author
Lookup NU author(s): Professor Jarka Glassey
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2024 by the authors. Large-scale bioprocesses are increasing globally to cater to the larger market demands for biological products. As fermenter volumes increase, the efficiency of mixing decreases, and environmental gradients become more pronounced compared to smaller scales. Consequently, the cells experience gradients in process parameters, which in turn affects the efficiency and profitability of the process. Computational fluid dynamics (CFD) simulations are being widely embraced for their ability to simulate bioprocess performance, facilitate bioprocess upscaling, downsizing, and process optimisation. Recently, CFD approaches have been integrated with dynamic Cell reaction kinetic (CRK) modelling to generate valuable information about the cellular response to fluctuating hydrodynamic parameters inside large production processes. Such coupled approaches have the potential to facilitate informed decision-making in intelligent biomanufacturing, aligning with the principles of “Industry 4.0” concerning digitalisation and automation. In this review, we discuss the benefits of utilising integrated CFD-CRK models and the different approaches to integrating CFD-based bioreactor hydrodynamic models with cellular kinetic models. We also highlight the suitability of different coupling approaches for bioprocess modelling in the purview of associated computational loads.
Author(s): Singh VK, Jimenez del Val I, Glassey J, Kavousi F
Publication type: Review
Publication status: Published
Journal: Bioengineering
Year: 2024
Volume: 11
Issue: 6
Online publication date: 27/05/2024
Acceptance date: 24/05/2024
ISSN (electronic): 2306-5354
Publisher: MDPI
URL: https://doi.org/10.3390/bioengineering11060546
DOI: 10.3390/bioengineering11060546
