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Lookup NU author(s): Dr Jie ZhangORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
This study aims to quantify haloperidol and methylparaben in a liquid pharmaceutical formulation (2 mg/ml) using UV spectrometry and the simultaneous equations method. Additionally, we explored the stability of haloperidol under various stress conditions. The UV analysis revealed maximum absorption peaks at 248 nm for haloperidol and 256 nm for methylparaben, using a 1% (v/v) lactic acid solution as the solvent. Method validation, conducted according to ICH guidelines, affirmed the method's reliability, showing excellent results in terms of linearity, precision, accuracy, and sensitivity. The method allows direct application to finished products, enabling simultaneous quantification without extractions. Its simplicity, speed, and cost-effectiveness make it ideal for routine controls in pharmaceutical industry haloperidol solution analyses. The method extends to monitoring forced degradation, indicating photolytic and hydrolytic degradation under acidic and basic conditions, while affirming thermal and oxidative stability. This proposed UV spectrometric method serves as a compelling alternative to pharmacopeia-recommended techniques, simplifying simultaneous determination of the active ingredient and preservative. This streamlines analysis, reducing time and costs. Additionally, it proves valuable in small industries lacking sophisticated instrumentation, offering insights into active ingredient behavior during forced degradation.
Author(s): Djilali K, Maachi R, Mesbah ZA, Nasrallah N, Touzout N, Tahraoui H, Zhang J, Amrane A
Publication type: Article
Publication status: Published
Journal: Analytical Biochemistry
Year: 2024
Volume: 695
Print publication date: 01/12/2024
Online publication date: 30/07/2024
Acceptance date: 28/07/2024
Date deposited: 01/08/2024
ISSN (print): 0003-2697
ISSN (electronic): 1096-0309
Publisher: Elsevier
URL: https://doi.org/10.1016/j.ab.2024.115632
DOI: 10.1016/j.ab.2024.115632
ePrints DOI: 10.57711/sfwj-ef95
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