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Lookup NU author(s): Dr Kathy StrattonORCiD
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DNA damage often causes an arrest of the cell cycle that provides time for genome integrity to be restored. In bacteria, the classical SOS DNA damage response leads to an inhibition of cell division resulting in temporarily filamentous growth. This raises the question as to whether such a response mechanism might similarly function in naturally filamentous bacteria such as Streptomyces. Streptomyces exhibit two functionally distinct forms of cell division: cross-wall formation in vegetative hyphae and sporulation septation in aerial hyphae. Here, we show that the genotoxic agent mitomycin C confers a block in sporulation septation in Streptomyces venezuelae in a mechanism that involves, at least in part, the downregulation of ssgB. Notably, this DNA damage response does not appear to block cross-wall formation and may be independent of canonical SOS and developmental regulators. We also show that the mitomycin C-induced block in sporulation can be partially bypassed by the constitutive expression of ssgB, though this appears to be largely limited to mitomycin C treatment and the resultant spore-like cells have reduced viability.
Author(s): Falguera JVT, Stratton KJ, Bush MJ, Jani C, Findlay KC, Schlimpert S, Nodwell JR
Publication type: Article
Publication status: Published
Journal: Microbiology
Year: 2022
Volume: 168
Issue: 6
Print publication date: 15/06/2022
Acceptance date: 09/05/2022
ISSN (print): 1350-0872
ISSN (electronic): 1465-2080
Publisher: Microbiology Society
URL: https://doi.org/10.1099/mic.0.001198
DOI: 10.1099/mic.0.001198
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