Toggle Main Menu Toggle Search

Open Access padlockePrints

Guidelines for minimal information on cellular senescence experimentation in vivo

Lookup NU author(s): Dr Mikolaj Ogrodnik, Professor Thomas von Zglinicki

Downloads


Licence

This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2024 The AuthorsCellular senescence is a cell fate triggered in response to stress and is characterized by stable cell-cycle arrest and a hypersecretory state. It has diverse biological roles, ranging from tissue repair to chronic disease. The development of new tools to study senescence in vivo has paved the way for uncovering its physiological and pathological roles and testing senescent cells as a therapeutic target. However, the lack of specific and broadly applicable markers makes it difficult to identify and characterize senescent cells in tissues and living organisms. To address this, we provide practical guidelines called “minimum information for cellular senescence experimentation in vivo” (MICSE). It presents an overview of senescence markers in rodent tissues, transgenic models, non-mammalian systems, human tissues, and tumors and their use in the identification and specification of senescent cells. These guidelines provide a uniform, state-of-the-art, and accessible toolset to improve our understanding of cellular senescence in vivo.


Publication metadata

Author(s): Ogrodnik M, Carlos Acosta J, Adams PD, d'Adda di Fagagna F, Baker DJ, Bishop CL, Chandra T, Collado M, Gil J, Gorgoulis V, Gruber F, Hara E, Jansen-Durr P, Jurk D, Khosla S, Kirkland JL, Krizhanovsky V, Minamino T, Niedernhofer LJ, Passos JF, Ring NAR, Redl H, Robbins PD, Rodier F, Scharffetter-Kochanek K, Sedivy JM, Sikora E, Witwer K, von Zglinicki T, Yun MH, Grillari J, Demaria M

Publication type: Review

Publication status: Published

Journal: Cell

Year: 2024

Volume: 187

Issue: 16

Pages: 4150-4175

Online publication date: 08/08/2024

Acceptance date: 02/04/2018

ISSN (print): 0092-8674

ISSN (electronic): 1097-4172

Publisher: Elsevier B.V.

URL: https://doi.org/10.1016/j.cell.2024.05.059

DOI: 10.1016/j.cell.2024.05.059


Share