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Amine-reactive crosslinking enhances type 0 collagen hydrogel properties for regenerative medicine

Lookup NU author(s): Dan Salthouse, Peter Goulding, Sophie Reay, Emma Jackson, Professor Katarina Novakovic, Professor Catharien Hilkens, Dr Ana Ferreira-DuarteORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

Copyright © 2024 Salthouse, Goulding, Reay, Jackson, Xu, Ahmed, Mearns-Spragg, Novakovic, Hilkens and Ferreira.Introduction: Collagen is extensively utilised in regenerative medicine due to its highly desirable properties. However, collagen is typically derived from mammalian sources, which poses several limitations, including high cost, potential risk of immunogenicity and transmission of infectious diseases, and ethical and religious constraints. Jellyfish-sourced type 0 collagen represents a safer and more environmentally sustainable alternative collagen source. Methods: Thus, we investigated the potential of jellyfish collagen-based hydrogels, obtained from Rhizostoma pulmo (R. pulmo) jellyfish, to be utilised in regenerative medicine. A variety of R. pulmo collagen hydrogels (RpCol hydrogels) were formed by adding a range of chemical crosslinking agents and their physicochemical and biological properties were characterised to assess their suitability for regenerative medicine applications. Results and Discussion: The characteristic chemical composition of RpCol was confirmed by Fourier-transform infrared spectroscopy (FTIR), and the degradation kinetics, morphological, and rheological properties of RpCol hydrogels were shown to be adaptable through the addition of specific chemical crosslinking agents. The endotoxin levels of RpCol were below the Food and Drug Administration (FDA) limit for medical devices, thus allowing the potential use of RpCol in vivo. 8-arm polyethylene glycol succinimidyl carboxyl methyl ester (PEG-SCM)-crosslinked RpCol hydrogels preserved the viability and induced a significant increase in the metabolic activity of immortalised human mesenchymal stem/stromal cells (TERT-hMSCs), therefore demonstrating their potential to be utilised in a wide range of regenerative medicine applications.


Publication metadata

Author(s): Salthouse D, Goulding PD, Reay SL, Jackson EL, Xu C, Ahmed R, Mearns-Spragg A, Novakovic K, Hilkens CMU, Ferreira AM

Publication type: Article

Publication status: Published

Journal: Frontiers in Bioengineering and Biotechnology

Year: 2024

Volume: 12

Pages: epub ahead of print

Online publication date: 26/07/2024

Acceptance date: 12/07/2024

Date deposited: 19/08/2024

ISSN (electronic): 2296-4185

Publisher: Frontiers Media SA

URL: https://doi.org/10.3389/fbioe.2024.1391728

DOI: 10.3389/fbioe.2024.1391728

Data Access Statement: The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://data.ncl.ac.uk.


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Funding

Funder referenceFunder name
Engineering and Physical Sciences Research Council (EPSRC) (grant number 2637800)
Engineering and Physical Sciences Research Council Doctoral Training Partnership (EPSRC DTP) scholarship (grant number 2442338)
Research into Inflammatory Arthritis Centre Versus Arthritis (RACE) (grant number 22072)

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