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Lookup NU author(s): Professor Giles Budge, Dr Hollie Pufal
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© 2024 International Bee Research Association.European foulbrood (EFB) is a severe bacterial honey bee brood disease caused by the Gram-positive bacterium Melissocccus plutonius. The disease is widely distributed worldwide, and is an increasing problem in some areas. Although the causative agent of EFB was described almost a century ago, many basic aspects of its pathogenesis are still unknown. Earlier studies were hampered by insensitive and unspecific methods such as culture based techniques. Recent advances in molecular technologies have led to a boom in the methods available to study both disease and causative organism, but not all published methods offer data of equal quality, or are likely to result in success. This paper presents selected step-by-step methodologies that have been used with success in at least one laboratory of the authors and considered relevant by the consortium of authors. We hope this paper helps providing some assistance to those wishing to work on M. plutonius and EFB, and speeds up the discovery of new knowledge to improve the control of this damaging and often neglected disease.
Author(s): Budge GE, Burns N, Takamatsu D, Erler S, Forsgren E, Grossar D, Hornitzky M, Milbrath M, Pufal H, Tomkies V, Wood S, Yordanova M, Charriere J-D
Publication type: Review
Publication status: Published
Journal: Journal of Apicultural Research
Year: 2024
Pages: epub ahead of print
Online publication date: 02/09/2024
Acceptance date: 18/06/2024
ISSN (print): 0021-8839
ISSN (electronic): 2078-6913
Publisher: Taylor and Francis Ltd.
URL: https://doi.org/10.1080/00218839.2024.2380425
DOI: 10.1080/00218839.2024.2380425
