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Lookup NU author(s): Dr Marco FusiORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Microbial communities respond to temperature with physiological adaptation and compositional turnover. Whether thermal selection of enzymes explains marine microbiome plasticity in response to temperature remains unresolved. By quantifying the thermal behaviour of seven functionally-independent enzyme classes (esterase, extradiol dioxygenase, phosphatase, beta-galactosidase, nuclease, transaminase, and aldo-keto reductase) in native proteomes of marine sediment microbiomes from the Irish Sea to the southern Red Sea, we record a significant effect of the mean annual temperature (MAT) on enzyme response in all cases. Activity and stability profiles of 228 esterases and 5 extradiol dioxygenases from sediment and seawater across 70 locations worldwide validate this thermal pattern. Modelling the esterase phase transition temperature as a measure of structural flexibility confirms the observed relationship with MAT. Furthermore, when considering temperature variability in sites with non-significantly different MATs, the broadest range of enzyme thermal behaviour and the highest growth plasticity of the enriched heterotrophic bacteria occur in samples with the widest annual thermal variability. These results indicate that temperature-driven enzyme selection shapes microbiome thermal plasticity and that thermal variability finely tunes such processes and should be considered alongside MAT in forecasting microbial community thermal response.
Author(s): Marasco R, Fusi M, Coscolín C, Barozzi A, Almendral D, Bargiela R, Gohlke C, Pfleger C, Dittrich J, Gohlke H, Matesanz R, Sanchez-Carrillo S, Mapelli F, Chernikova TN, Golyshin PN, Ferrer M, Daffonchio D
Publication type: Article
Publication status: Published
Journal: Nature Communications
Year: 2023
Volume: 14
Online publication date: 24/02/2023
Acceptance date: 08/02/2023
Date deposited: 18/10/2024
ISSN (electronic): 2041-1723
Publisher: Nature Publishing Group
URL: https://doi.org/10.1038/s41467-023-36610-0
DOI: 10.1038/s41467-023-36610-0
Data Access Statement: The authors declare that the main data supporting the findings of this study are available within the paper and related Supplementary Information, Supplementary Data and Source Data files. Accession numbers to retrieve metagenomes analysed in this study are reported in Supplementary Table S3, Supplementary Data S3 and Supplementary Data S4 files. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD039714. The data related to Tp have been deposited at researchdata.hhu.de under the identifier [https://doi.org/10.25838/d5p-42] 101. To use the archive, download the file, remove the .txt ending, and use WinRAR, for example, to open the archive. Microbiome sequences extracted from temperature variability sediments and related enriched heterotrophic bacteria were deposited in the NCBI database under the SRA accession number PRJNA508596. Source data are provided with this paper.
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