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Structure of the RNA-dependent RNA polymerase P2 from the cystovirus φ8

Lookup NU author(s): Professor Paula SalgadoORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

© The Author(s) 2024.The replication of RNA viruses relies on the activity of RNA-dependent RNA polymerases (RdRps). Despite large variations in their genomic sequences, viral RdRps share a common architecture generally known as a closed right hand. The P2 polymerase of cystovirus φ6 is currently among the best characterized viral RdRps. This polymerase is responsible for carrying out both replication and transcription of the viral double-stranded RNA genome using de novo initiation. Despite the extensive biochemical and structural studies conducted on φ6 P2, further structural information on other cystoviral RdRps is crucial to elucidate the structural and functional diversity of viral RdRps. Here, we have determined the atomic X-ray structure of the RdRp P2 from the φ6-related cystovirus φ8 at 3Å resolution. This structure completes the existing set of structural information on the φ8 polymerase complex and sheds light on the difference and similarities with related cystoviral RdRps.

Publication metadata

Author(s): Latimer-Smith M, Salgado PS, Forsyth I, Makeyev E, Poranen MM, Stuart DI, Grimes JM, El Omari K

Publication type: Article

Publication status: Published

Journal: Scientific Reports

Year: 2024

Volume: 14

Issue: 1

Online publication date: 09/10/2024

Acceptance date: 03/10/2024

Date deposited: 23/10/2024

ISSN (electronic): 2045-2322

Publisher: Nature Research

URL: https://doi.org/10.1038/s41598-024-75213-7

DOI: 10.1038/s41598-024-75213-7

Data Access Statement: The structure factors and coordinates for f8 P2 have been deposited in the Protein Data Bank (https://www.rcsb.org/) under the accession code 9FEJ.

PubMed id: 39384884

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