Browse by author
Lookup NU author(s): Dr Kheng-Lim GohORCiD, Dr Vladimir Zivkovic
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
© 2024 Elsevier Ltd. Flavor esters are used in food and cosmetic industries, but sustainable production remains a big challenging. This study proposed the ultrasound-assisted biosynthesis of cinnamyl acetate using self-made immobilized lipase CSL@OMS-C8, with computational fluid dynamics (CFD) and molecular simulations revealing the hydrodynamic properties and lipase-catalytic mechanisms. The results demonstrate that ultrasonication-intensified enzymatic reaction facilitated 96.6 % conversion of cinnamic alcohol, due to the ultrasound-assisted catalytic efficiency of 13.7 mmol/g·min. Molecular docking analysis identifies the lowest binding energy of −3.7 kcal·mol−1 between lipase and vinyl acetate, contributing to the highest conversion rates compared to acetic acid, ethyl acetate. CFD simulation indicates that ultrasonic energy waves promote substrate diffusion and mixing with lower shear stress. The catalytic stability of CSL@OMS-C8 was confirmed by 60.1 % of relative activity after 10-time reuse. This paper theoretically and experimentally studied the ultrasonic-assisted enzymatic synthesis of cinnamyl acetate, showcasing huge potential for sustainable production of flavor esters.
Author(s): Chen J, Liu T, Zhang Y, Zheng L, Goh K-L, Zivkovic V, Zheng M
Publication type: Article
Publication status: Published
Journal: Food Chemistry
Year: 2025
Volume: 464
Print publication date: 01/02/2025
Online publication date: 01/11/2024
Acceptance date: 28/10/2024
ISSN (print): 0308-8146
ISSN (electronic): 1873-7072
Publisher: Elsevier Ltd
URL: https://doi.org/10.1016/j.foodchem.2024.141843
DOI: 10.1016/j.foodchem.2024.141843
Altmetrics provided by Altmetric
