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Lookup NU author(s): Dr Fiona MalcomsonORCiD, Dr Bano Louca, Dr Naomi Willis, Dr Long Xie, Professor Bernard Corfe, Professor Christopher StewartORCiD, Professor John Mathers
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© The Authors 2024.The gut microbiome is impacted by certain types of dietary fibre. However, the type, duration, and dose needed to elicit gut microbial changes, and whether these changes also influence microbial metabolites, remains unclear. This study investigated the effects of supplementing healthy participants with two types of non-digestible carbohydrates (resistant starch (RS) and polydextrose (PD)), on the stool microbiota and microbial metabolite concentrations in plasma, stool, and urine, as secondary outcomes in the Dietary Intervention Stem Cells and Colorectal Cancer (DISC) Study. The DISC Study was a double-blind, randomised controlled trial that supplemented healthy participants with RS and/or PD or placebo for 50 days in a 2*2 factorial design. DNA was extracted from stool samples collected pre- and post-intervention, and V4 16S rRNA gene sequencing was used to profile the gut microbiota. Metabolite concentrations were measured in stool, plasma, and urine by high-performance liquid chromatography. A total of 58 participants with paired samples available were included. After 50 days, no effects of RS or PD were detected on composition of the gut microbiota diversity (alpha- and beta-diversity), on genus relative abundance, or on metabolite concentrations. However, Drichlet’s multinomial mixture clustering-based approach suggests that some participants changed microbial enterotype post-intervention. The gut microbiome and faecal, plasma, and urinary microbial metabolites were stable in response to a 50-day fibre intervention in middle aged adults. Larger and longer studies, including those which explore the effects of specific fibre sub-types, may be required to determine the relationships between fibre intake, the gut microbiome, and host-health.
Author(s): Malcomson FC, Louca P, Nelson A, Willis ND, McCallum I, Xie L, Ouwehand AC, Stowell JD, Preston T, Morrison DJ, Kelly SB, Michael Bradburn D, Belshaw NJ, Johnson IT, Corfe BM, Stewart CJ, Mathers JC
Publication type: Article
Publication status: Published
Journal: British Journal of Nutrition
Year: 2024
Pages: epub ahead of print
Online publication date: 04/11/2024
Acceptance date: 02/04/2018
ISSN (print): 0007-1145
ISSN (electronic): 1475-2662
Publisher: Cambridge University Press
URL: https://doi.org/10.1017/S000711452400271X
DOI: 10.1017/S000711452400271X
PubMed id: 39494600
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