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Application of single-cell transcriptomics to kinetoplastid research

Lookup NU author(s): Dr Emma BriggsORCiD

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0).

Abstract

Kinetoplastid parasites are responsible for both human and animal diseases across the globe where they have a great impact on health and economic well-being. Many species and life cycle stages are difficult to study due to limitations in isolation and culture, as well as to their existence as heterogeneous populations in hosts and vectors. Single-cell transcriptomics (scRNA-seq) has the capacity to overcome many of these difficulties, and can be leveraged to disentangle heterogeneous populations, highlight genes crucial for propagation through the life cycle, and enable detailed analysis of host-parasite interactions. Here, we provide a review of studies that have applied scRNA-seq to protozoan parasites so far. In addition, we provide an overview of sample preparation and technology choice considerations when planning scRNA-seq experiments, as well as challenges faced when analysing the large amounts of data generated. Finally, we highlight areas of kinetoplastid research that could benefit from scRNA-seq technologies.

Publication metadata

Author(s): Briggs EM, Warren FSL, Matthews KR, McCulloch R, Otto TD

Publication type: Article

Publication status: Published

Journal: Parasitology

Year: 2021

Volume: 148

Issue: 10

Pages: 1223-1236

Print publication date: 01/09/2021

Online publication date: 08/03/2021

Acceptance date: 24/02/2021

Date deposited: 20/02/2025

ISSN (print): 0031-1820

ISSN (electronic): 1469-8161

Publisher: Cambridge University Press

URL: https://doi.org/10.1017/S003118202100041X

DOI: 10.1017/S003118202100041X

PubMed id: 33678213

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