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Lookup NU author(s): Dr Katherine DuncanORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2016 by the authors; licensee MDPI, Basel, Switzerland. The oceans represent an understudied resource for the isolation of bacteria with the potential to produce novel secondary metabolites. In particular, actinomyces are well known to produce chemically diverse metabolites with a wide range of biological activities. This study characterised spore-forming bacteria from both Scottish and Antarctic sediments to assess the influence of isolation location on secondary metabolite production. Due to the selective isolation method used, all 85 isolates belonged to the phyla Firmicutes and Actinobacteria, with the majority of isolates belonging to the genera Bacillus and Streptomyces. Based on morphology, thirty-eight isolates were chosen for chemical investigation. Molecular networking based on chemicaL profiles (HR-MS/MS) of fermentation extracts was used to compare complex metabolite extracts. The results revealed 40% and 42% of parent ions were produced by Antarctic and Scottish isolated bacteria, respectively, and only 8% of networked metabolites were shared between these locations, implying a high degree of biogeographic influence upon secondary metabolite production. The resulting molecular network contained over 3500 parent ions with a mass range of m/z 149–2558 illustrating the wealth of metabolites produced. Furthermore, seven fermentation extracts showed bioactivity against epithelial colon adenocarcinoma cells, demonstrating the potential for the discovery of novel bioactive compounds from these understudied locations.
Author(s): Purves K, Macintyre L, Brennan D, Hreggvidsson GO, Kuttner E, Asgeirsdottir ME, Young LC, Green DH, Edrada-Ebel R, Duncan KR
Publication type: Article
Publication status: Published
Journal: Metabolites
Year: 2016
Volume: 6
Issue: 1
Online publication date: 08/01/2016
Acceptance date: 30/12/2015
Date deposited: 15/01/2025
ISSN (electronic): 2218-1989
Publisher: MDPI
URL: https://doi.org/10.3390/metabo6010002
DOI: 10.3390/metabo6010002
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