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Lookup NU author(s): Dr Changhyun LimORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2025 The Author(s). Experimental Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.Understanding the turnover of proteins in tissues gives information as to how external stimuli result in phenotypic change. Nowhere is such phenotypic change more conspicuous than skeletal muscle, which can be effectively remodelled by increased loading, ageing and unloading (disuse), all of which are subject to modification by nutrition and other environmental stimuli. The understanding of muscle proteome remodelling has undergone a renaissance recently with the reintroduction of deuterated water (D2O) and its ingestion to label amino acids and measure their incorporation into proteins. However, there is confusion around the use of the deuterated water methodology and the interpretation of the data it provides. Here, we provide a short review of some of the more salient features of the method and clarify some of the confusion around the method of deuterated water methods and its use in humans and how the interpretation of the data is in contrast to that of rodents.
Author(s): Lim C, McKendry J, Lees M, Atherton PJ, Burd NA, Holwerda AM, van Loon LJC, McGlory C, Mitchell CJ, Smith K, Wilkinson DJ, Stokes T, Phillips SM
Publication type: Review
Publication status: Published
Journal: Experimental Physiology
Year: 2025
Pages: epub ahead of print
Online publication date: 05/02/2025
Acceptance date: 03/01/2025
ISSN (print): 0958-0670
ISSN (electronic): 1469-445X
Publisher: John Wiley and Sons Inc
URL: https://doi.org/10.1113/EP092353
DOI: 10.1113/EP092353
