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Overexpression of sucrose phosphate synthase increases sucrose unloading in transformed tomato fruit

Lookup NU author(s): Mimi Nguyen, Professor Christine Foyer

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Abstract

Sucrose unloading and sink activity were examined in tomato plants (Lycopersicon esculentum) overexpressing sucrose phosphate synthase (SPS; EC 2.3.1.14). Like the leaves, the fruit of the transformed tomato plants had elevated (2.4-fold) SPS activity. SPS over-expression in tomato fruit did not significantly change acid invertase, and only slightly reduced ADPglc ppase activity, but enhanced sucrose synthase activity by 27%. More importantly, the amount of sucrose unloaded into the fruit was considerably increased. Using [3H]- (fructosyl)-sucrose in in vitro unloading experiments with harvested 20-d-old fruit, 70% more sucrose was unloaded into the transformed fruits compared to the untransformed controls. Furthermore, the turnover of the sucrose unloaded into the fruit of transformed plants was 60% higher than that observed in the untransformed controls. Taken together, these results demonstrate that SPS overexpression increases the sink strength of transformed tomato fruit.

Publication metadata

Author(s): Nguyen-Quoc B, N'Tchobo H, Foyer CH, Yelle S

Publication type: Article

Publication status: Published

Journal: Journal of Experimental Botany

Year: 1999

Volume: 50

Issue: 335

Pages: 785-791

Print publication date: 01/06/1999

ISSN (print): 0022-0957

ISSN (electronic): 1460-2431

Publisher: Oxford University Press

URL: http://dx.doi.org/10.1093/jxb/50.335.785

DOI: 10.1093/jxb/50.335.785

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