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Lookup NU author(s): Dr Christin AlbusORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2025 The Author(s)Neuronal growth and regeneration are regulated by local translation of mRNAs in axons. We examined RNA polyadenylation changes upon sensory neuron injury and found upregulation of a subset of polyadenylated B2-SINE repeat elements, hereby termed GI-SINEs (growth-inducing B2-SINEs). GI-SINEs are induced from ATF3 and other AP-1 promoter-associated extragenic loci in injured sensory neurons but are not upregulated in lesioned retinal ganglion neurons. Exogenous GI-SINE expression elicited axonal growth in injured sensory, retinal, and corticospinal tract neurons. GI-SINEs interact with ribosomal proteins and nucleolin, an axon-growth-regulating RNA-binding protein, to regulate translation in neuronal cytoplasm. Finally, antisense oligos against GI-SINEs perturb sensory neuron outgrowth and nucleolin-ribosome interactions. Thus, a specific subfamily of transposable elements is integral to a physiological circuit linking AP-1 transcription with localized RNA translation.
Author(s): Zahavi EE, Koppel I, Kawaguchi R, Oses-Prieto JA, Briner A, Monavarfeshani A, Dalla Costa I, van Niekerk E, Lee J, Matoo S, Hegarty S, Donahue RJ, Sahoo PK, Ben-Dor S, Feldmesser E, Ryvkin J, Leshkowitz D, Perry RB-T, Cheng Y, Farber E, Abraham O, Samra N, Okladnikov N, Alber S, Albus CA, Rishal I, Ulitsky I, Tuszynski MH, Twiss JL, He Z, Burlingame AL, Fainzilber M
Publication type: Article
Publication status: Published
Journal: Cell
Year: 2025
Volume: 188
Issue: 16
Pages: p4350-4365.e22
Online publication date: 16/05/2025
Acceptance date: 23/04/2025
Date deposited: 28/05/2025
ISSN (print): 0092-8674
ISSN (electronic): 1097-4172
Publisher: Elsevier B.V.
URL: https://doi.org/10.1016/j.cell.2025.04.030
DOI: 10.1016/j.cell.2025.04.030
Data Access Statement: RNA-seq datasets are available from the NCBI GEO database under accession numbers GEO: GSE231596 and GEO: GSE279906. Proteomics data are available from the ProteomeXchange PRIDE partner repository under dataset identifier PRIDE: PXD060976. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon reasonable request. This paper does not report original code.
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