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Lookup NU author(s): Dr Christin AlbusORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
Importin β1 (KPNB1) is a nucleocytoplasmic transport factor with critical roles in both cytoplasmic and nucleocytoplasmic transport, hence there is keen interest in the characterization of its subcellular interactomes. We found limited efficiency of BioID in the detection of importin complex cargos and therefore generated a highly specific and sensitive anti-KPNB1 monoclonal antibody to enable biotinylation by antibody recognition analysis of importin β1 interactomes. The monoclonal antibody recognizes an epitope comprising residues 301-320 of human KPBN1 and strikingly is highly specific for cytoplasmic KPNB1 in diverse applications, with little reaction with KPNB1 in the nucleus. Biotinylation by antibody recognition with this novel antibody revealed numerous new interactors of importin β1, expanding the KPNB1 interactome to cytoplasmic and signaling complexes that highlight potential new functions for the importins complex beyond nucleocytoplasmic transport. Data are available via ProteomeXchange with identifier PXD032728.
Author(s): Song DA, Alber S, Doron-Mandel E, Schmid V, Albus CA, Leitner O, Hamawi H, Oses-Prieto JA, Dezorella N, Burlingame AL, Fainzilber M, Rishal I
Publication type: Article
Publication status: Published
Journal: Molecular & Cellular Proteomics
Year: 2022
Volume: 21
Issue: 11
Print publication date: 01/11/2022
Online publication date: 28/09/2022
Acceptance date: 20/09/2022
Date deposited: 26/06/2025
ISSN (print): 1535-9476
ISSN (electronic): 1535-9484
Publisher: American Society for Biochemistry and Molecular Biology Inc.
URL: https://doi.org/10.1016/j.mcpro.2022.100418
DOI: 10.1016/j.mcpro.2022.100418
Data Access Statement: The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE (57) partner repository with the dataset identifier PXD032728. The mAbKPNB1-301-320 antibody will be available through the Ximbio repository (https://ximbio.com/). Data are available via ProteomeXchange with identifier PXD032728.
PubMed id: 36180036
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