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Lookup NU author(s): Professor Anthony De SoyzaORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Copyright ©The authors 2025.Background The European Multicentre Bronchiectasis Audit and Research Collaboration (EMBARC) registry shows considerable variation in culturable microbes in sputum between different European countries. The additive role of next-generation metagenomic sequencing remains unexplored and the association with antimicrobial resistomes unknown. Methods We used next-generation shotgun metagenomic sequencing to prospectively assess sputum from 349 individuals recruited into the EMBARC Bronchiectasis Research Involving Databases, Genomics and Endotyping (BRIDGE) study from three European regions: Northern and Western Europe, Southern Europe and the UK. Samples were included from eight European countries. Microbiome and resistome profiles were assessed in relation to clinical outcomes. Results Next-generation metagenomic sequencing reproduced differences between countries in microbial profiles that were previously shown by culture in the EMBARC study. Metagenomics provided enhanced detection for some bronchiectasis pathogens, including Pseudomonas aeruginosa, Haemophilus influenzae and Streptococcus pneumoniae. Three metagenomic microbial clusters dominated by the genera Pseudomonas, Streptococcus and Haemophilus demonstrated pan-European but variable distribution. Diverse resistomes, linked to underlying microbiomes, were identified across Europe, with significantly higher diversity of resistance gene determinants in Southern Europe. Resistome composition significantly differed between regions, characterised by regionally contrasting multidrug-resistant profiles. The EMBARC-BRIDGE cohort validated established bronchiectasis resistotypes RT1 and RT2, which occur at varying frequency across regions. Despite geographic variation in microbiome and resistome profiles in bronchiectasis across Europe, analogous antimicrobial resistance gene profiles associated with the key bronchiectasis genera Pseudomonas, Streptococcus and Haemophilus, independent of country or region. Conclusion Sputum metagenomics confirms and extends prior observations of regional variation in bronchiectasis microbiology. Important variation in the distribution of pathogens and antimicrobial resistance genes has implications for antimicrobial practices across Europe.
Author(s): Thng KX, Tiew PY, Aogain MM, Narayana JK, Jaggi TK, Ivan FX, Shuttleworth M, Long MB, Richardson H, Lind H, de Lima Headley DA, Robertson K, Pollock J, Goeminne PC, Shteinberg M, De Soyza A, Aliberti S, Altenburg J, Haworth CS, Sibila O, Polverino E, Loebinger MR, Ringshausen FC, Lorent N, Dimakou K, Shoemark A, Chalmers JD, Chotirmall SH
Publication type: Article
Publication status: Published
Journal: European Respiratory Journal
Year: 2025
Volume: 66
Issue: 2
Print publication date: 01/08/2025
Online publication date: 22/08/2025
Acceptance date: 28/04/2025
Date deposited: 09/12/2025
ISSN (print): 0903-1936
ISSN (electronic): 1399-3003
Publisher: European Respiratory Society
URL: https://doi.org/10.1183/13993003.00054-2025
DOI: 10.1183/13993003.00054-2025
PubMed id: 40404216
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