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The structure and proinflammatory activity of the lipopolysaccharide from Burkholderia multivorans and the differences between clonal strains colonizing pre and posttransplanted lungs

Lookup NU author(s): Dr Anjam Khan, Professor Kate Gould, Emeritus Professor Nick Europe-Finner, Professor Anthony De SoyzaORCiD, Professor Antonio Molinaro


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The Burkholderia cepacia complex is a group of Gram-negative bacteria that are opportunistic pathogens for humans especially in cystic fibrosis patients. Lipopolysaccharide (LPS) molecules are potent virulence factors of Gram-negative bacteria organisms essential for bacterial survival. A complete analysis of the bacterial lipopolysaccharide structure to function relationship is required to understand the chemical basis of the inflammatory process. We have therefore investigated the structures of lipopolysaccharides from clonally identical Burkholderia multivorans strains (genomovar II) isolated pre- and post-lung transplantation through compositional analysis, mass spectrometry, and 2D NMR spectroscopy. We tested the LPS proinflammatory activity as a stimulant of human myelomonocytic U937 cell cytokine induction and assessed TLR4/MD2 signaling. Marked changes between the paired strains were found in the lipid A-inner core region. Such structural variations can contribute to the bacterial survival and persistence of infections despite the loss of a CF milieu following lung transplantation. PMID: 18684854 [PubMed - indexed for MEDLINE]

Publication metadata

Author(s): IeranĂ² T, Silipo A, Sturiale L, Garozzo D, Brookes H, Khan CM, Bryant C, Gould FK, Corris PA, Lanzetta R, Parrilli M, De Soyza A, Molinaro A

Publication type: Article

Publication status: Published

Journal: Glycobiology

Year: 2008

Volume: 18

Issue: 11

Pages: 871-881

ISSN (print): 0959-6658

ISSN (electronic): 1460-2423

Publisher: Oxford University Press


DOI: 10.1093/glycob/cwn074


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Funder referenceFunder name
FFC 11/2006Italian Cystic Fibrosis Research Foundation