Browse by author
Lookup NU author(s): Dr Simon BamforthORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Developmental malformations of the heart in mouse embryos are commonly studied by histological sectioning. This is slow, labour intensive, and results in the loss of three-dimensional (3D) information. Magnetic resonance studies of embryos typically use spin-echo sequences, using prolonged acquisition times (>36 h) or perfusion with contrast agents to enhance resolution and contrast. This is technically difficult, and requires significant amounts of operator time. We imaged paraformaldehyde fixed embryos using a fast spoiled 3D gradient echo sequence with T(1)-weighting, in unattended overnight runs of less than 9 h. In wild-type embryos, we visualised normal cardiac structures, including cardiac chambers, the ventricular septum, primary and secondary atrial septa, valves, superior and inferior vena cava, aorta, pulmonary artery, and ductus arteriosus. In embryos lacking Cited2 (a transcriptional co-activator required for normal heart development), we identified cardiac malformations including atrial and ventricular septal defects, cono-truncal defects, and aortic arch malformations. We generated 3D reconstructions of normal and mutant hearts using contour identification and surface rendering computer software. The malformations were confirmed by histological sectioning. Our data indicate that fast gradient echo sequence magnetic resonance imaging can be used to rapidly and accurately identify complex cardiovascular malformations in transgenic and mutant mouse embryos.
Author(s): Bamforth SD; Schneider JE; Farthing CR; Clarke K; Neubauer S; Bhattacharya S
Publication type: Article
Publication status: Published
Journal: Journal of Molecular and Cellular Cardiology
Year: 2003
Volume: 35
Issue: 2
Pages: 217-222
ISSN (print): 0022-2828
ISSN (electronic): 1095-8584
Publisher: Academic Press
URL: http://dx.doi.org/10.1016/S0022-2828(02)00291-2
DOI: 10.1016/S0022-2828(02)00291-2
Notes: Evaluation Studies Journal Article
Altmetrics provided by Altmetric
