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Energetic stress induces premature aging of diploid human fibroblasts (Wi-38) in vitro

Lookup NU author(s): Dr Julia Reichelt


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Oxidative phosphorylation is the main endogenous source for the generation of reactive oxygen species (ROS). In order to investigate the influence of enhanced ROS production on the in vitro senescence of Wi-38 fibroblasts, cells were cultivated in medium with elevated (hypertonic) NaCl concentrations. The number of active Na(+)/K(+)-ATPase molecules per cell was found to be increased. A rise in both respiration and glycolysis as evidenced by the increases in oxygen and glucose consumption and lactate production was revealed. Cells stayed alive in medium with NaCl concentrations of up to 0.30 M and could be adapted to growth under these hypertonic conditions (high-NaCl tolerant cells). These cells exhibited an increased cell size and protein content. A growing number of cells showed stress fibers and granulation. The proliferation rate and the maximum number of cumulative population doublings of these high-NaCl tolerant cultures were reduced and saturation density was decreased. Thus, these cells under energetic stress due to increased energy requirements for active ion transport expressed features typical for aging in vitro. We conclude therefore that energetic stress induces premature aging in human diploid fibroblasts.

Publication metadata

Author(s): Reichelt J; Schachtschabel DO

Publication type: Article

Publication status: Published

Journal: Archives of Gerontology and Geriatrics

Year: 2001

Volume: 32

Issue: 3

Pages: 219-231

ISSN (print): 0167-4943

ISSN (electronic): 1872-6976

Publisher: Elsevier Ireland Ltd


DOI: 10.1016/S0167-4943(01)00102-9



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