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A critique of avian CHD-based molecular sexing protocols illustrated by a Z-chromosome polymorphism detected in auklets

Lookup NU author(s): Dr Steven Darby

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Abstract

The sexes of non-ratite birds can be determined routinely by PCR amplification of the CHD-Z and CHD-W genes. CHD-based molecular sexing of four species of auklets revealed the presence of a polymorphism in the Z chromosome. No deviation from a 1:1 sex ratio was observed among the chicks, though the analyses were of limited power. Polymorphism in the CHD-Z gene has not been reported previously in any bird, but if undetected it could lead to the incorrect assignment of sex. We discuss the potential difficulties caused by a polymorphism such as that identified in auklets and the merits of alternative CHD-based sexing protocols and primers.

Publication metadata

Author(s): Dawson DA, Darby S, Hunter FM, Krupa AP, Jones IL, Burke T

Publication type: Article

Publication status: Published

Journal: Molecular Ecology Notes

Year: 2001

Volume: 1

Issue: 3

Pages: 201-204

ISSN (print): 1471-8278

ISSN (electronic): 1755-0998

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1046/j.1471-8278.2001.00060.x

DOI: 10.1046/j.1471-8278.2001.00060.x

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