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Lookup NU author(s): Dr Meryl Lusher, Dr Sam Richmond
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This paper describes the isolation and characterisation of a strain of Chlamydia psittaci obtained from a nasal swab taken from a horse with serous nasal discharge. Initial isolation was achieved in cycloheximide-treated McCoy cell monolayers. Chlamydial inclusions stained by immunofluorescence either with a rabbit antiserum raised against C. psittaci or with a monoclonal antibody directed against the genus-specific lipopolysaccharide antigen were single and compact. They did not stain with iodine or with a monoclonal antibody reactive against Chlamydia trachomatis. The agent was re-isolated in the yolk sacs of embryonated hens eggs and designated N16. Identification of the agent was confirmed by electron microscopy. Unique plasmid DNA was prepared from a purified suspension of chlamydial elemenrary bodies (EBs), and analysed by electrophoresis through 1.0% agarose gels stained by ethidium bromide. This strain of C. psittaci grew relatively slowly in cycloheximide-treated McCoy cells, and the yield of elementary bodies during the course of one growth cycle was relatively low.
Author(s): Wills JM, Watson G, Lusher ME, Mair TS, Wood D, Richmond SJ
Publication type: Article
Publication status: Published
Journal: Veterinary Microbiology
Year: 1990
Volume: 24
Issue: 1
Pages: 11-19
Print publication date: 01/07/1990
ISSN (print): 0378-1135
ISSN (electronic): 1873-2542
Publisher: Elsevier BV
URL: http://dx.doi.org/10.1016/0378-1135(90)90046-X
DOI: 10.1016/0378-1135(90)90046-X
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