Toggle Main Menu Toggle Search

Open Access padlockePrints

Deficiency of C4 from donor or recipient mouse fails to prevent renal allograft rejection

Lookup NU author(s): Professor Neil Sheerin

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Complement effector products generated in the transplanted kidney are known to mediate transplant rejection, but which of the three main activation pathways of complement trigger this response is unclear. Here we assessed the role of the classical and lectin pathways by studying the common component C4 in mouse kidney transplant rejection. We transplanted wild-type or C4-null H-2(b) donor kidneys into H-2(k) or H-2(d) recipients, or vice-versa, to assess the roles of donor kidney and recipient expression of complement. Intragraft C4 gene expression rose substantially during rejection. However, we found no significant association between graft acceptance and the presence of C4 in either the donor kidney or recipient mouse. At the time of rejection, we found no significant differences in alloantibody response in the different groups. Tubular deposition of C3 to C9 occurred regardless of the absence or presence of C4 in either the donor or recipient mouse, indicating that C4 was dispensable for complement activation at this site. These data suggest that complement activation and renal allograft rejection are independent of the classical and lectin pathways in these models, implying that in the absence of these pathways the alternative pathway is the main trigger for complement-mediated rejection.


Publication metadata

Author(s): Lin T, Zhou W, Farrar CA, Hargreaves RE, Sheerin NS, Sacks SH

Publication type: Article

Publication status: Published

Journal: American Journal of Pathology

Year: 2006

Volume: 168

Issue: 4

Pages: 1241-1248

ISSN (print): 0002-9440

ISSN (electronic): 1525-2191

Publisher: American Society for Investigative Pathology

URL: http://dx.doi.org/10.2353/ajpath.2006.050360

DOI: 10.2353/ajpath.2006.050360

Notes: Journal Article Research Support, Non-U.S. Gov't United States


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share