Toggle Main Menu Toggle Search

Open Access padlockePrints

Myotoxic effects of mastoparan from Polybia paulista (Hymenoptera, Epiponini) wasp venom in mice skeletal muscle

Lookup NU author(s): Thalita Rocha


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


In a previous study, we showed that the Polybia paulista wasp venom causes strong myonecrosis. This study was undertaken to characterize the myotoxic potency of mastoparan (Polybia-MPII) isolated from venom (0.25 μg/μl) and injected in the tibial anterior (TA) muscle (i.m.) of Balb/c mice. The time course of the changes was followed at muscle degenerative (3 and 24 h) and regenerative (3, 7, and 21 days) periods (n=6) after injection and compared to matched controls by calculation of the percentage of cross-sectional area affected and determination of creatine kinase (CK) activity (n=10). The results showed that although MP was strongly myotoxic, its capacity for regeneration was maintained high. Since the extent of tissue damage was not correlated with the CK serum levels, which remained very low, we raised the hypothesis that the enzyme underwent denaturation by the peptide. Evidence suggested that MP induced the death of TA fibers by necrosis and apoptosis and had the sarcolemma as its primordial target. Given its amphiphilic polycationic nature and based on the vast spectrum of functions attributed to the peptide, we suggest that MP interaction with cell membrane impaired the phosphorylation of dystrophin essential for sarcolemma mechanical stability, and disturbed Ca2+ mobilization with obvious implications on sarcoplasmic reticulum and mitochondrial functioning.

Publication metadata

Author(s): Rocha T, Souza BM, Palma MS, Cruz-Hofling MA

Publication type: Article

Publication status: Published

Journal: Toxicon

Year: 2007

Volume: 50

Issue: 5

Pages: 589-599

ISSN (print): 0041-0101

ISSN (electronic): 1879-3150

Publisher: Pergamon


DOI: 10.1016/j.toxicon.2007.05.003


Altmetrics provided by Altmetric