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Significant contribution of the pgdA gene to the virulence of Streptococcus suis

Lookup NU author(s): Dr Nhat Khai Bui, Professor Waldemar Vollmer


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Streptococcus suis is a major swine pathogen and emerging zoonotic agent. In this study we have determined the muropeptide composition of S. suis peptidoglycan (PG) and found, among other modifications, N-deacetylated compounds. Comparison with an isogenic mutant showed that the product of the pgdA gene is responsible for this specific modification which occurred in very low amounts. Low level of PG N-deacetylation correlated with absence of significant lysozyme resistance when wild-type S. suis was grown in vitro. On the other hand, expression of the pgdA gene was increased upon interaction of the bacterium with neutrophils in vitro as well as in vivo in experimentally inoculated mice, suggesting that S. suis may enhance PG N-deacetylation under these conditions. Evaluation of the ΔpgdA mutant in both the CD1 murine and the porcine models of infection revealed a significant contribution of the pgdA gene to the virulence traits of S. suis. Reflecting a severe impairment in its ability to persist in blood and decreased ability to escape immune clearance mechanisms mediated by neutrophils, the ΔpgdA mutant was highly attenuated in both models. The results of this study suggest that modification of PG by N-deacetylation is an important factor in S. suis virulence.

Publication metadata

Author(s): Fittipaldi N, Sekizaki T, Takamatsu D, de la Cruz Domínguez-Punaro M, Harel J, Bui NK, Vollmer W, Gottschalk M

Publication type: Article

Publication status: Published

Journal: Molecular Microbiology

Year: 2008

Volume: 70

Issue: 5

Pages: 1120-1135

ISSN (print): 0950-382X

ISSN (electronic): 1365-2958

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1111/j.1365-2958.2008.06463.x


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Funder referenceFunder name
Centre de Recherche en Infectiologie Porcine (CRIP-FQRNT)
0680154280Natural Sciences and Engineering Research Council of Canada
LSHM-CT-2004512138European Commission