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The soluble sperm factor that causes Ca2+ release from sea-urchin (Lytechinus pictus) egg homogenates also triggers Ca2+ oscillations after injection into mouse eggs

Lookup NU author(s): Professor Keith Jones

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Abstract

Cytosolic extracts of boar sperm contain a soluble phospholipase C (PLC) activity that induces Ca²⁺ release in sea-urchin (Lytechinus pictus) egg homogenates and an uncharacterized protein factor that causes Ca²⁺ oscillations when injected into mammalian eggs. In the present study we fractionated boar sperm extracts on three different FPLC chromatographic columns and found that the fractions that caused maximal Ca²⁺ release in sea-urchin egg homogenates were also the ones that triggered Ca²⁺ oscillations in mouse eggs. Our data suggests that the sperm factor which triggers Ca²⁺ oscillations in eggs contains a PLC and not the 33 kDa glucosamine deaminase previously suggested to be one its components.

Publication metadata

Author(s): Parrington J, Jones KT, Lai A, Swann K

Publication type: Article

Publication status: Published

Journal: Biochemical Journal

Year: 1999

Volume: 341

Issue: 1

Pages: 1-4

Print publication date: 24/06/1999

ISSN (print): 0264-6021

ISSN (electronic): 1470-8728

Publisher: Portland Press Ltd.

URL: http://www.biochemj.org/bj/341/0001/3410001.pdf

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